When performing chromatography experiments, the use of chromatography vials is essential for successful analysis. These small vials hold a substance securely, preventing adsorption and leaching. The proper use of vials ensures that samples are protected from contaminants, resulting in improved productivity within the facility. By understanding the materials used to make chromatography vials, you can better choose the right ones for your specific needs. Learn more about vials by reading this article. Chromatography vials are available in glass and plastic varieties. The use of glass vials allows the sample to be protected from ultraviolet (UV) light, which can damage certain chemicals. Also, glass is heat-resistant and can withstand temperatures over 500 degrees Celsius. These advantages make glass vials a common choice for many laboratories. A high-quality glass vial can be easily cleaned and sterilized. Glass is the preferred choice for the majority of chromatography experiments, but you can also choose amber or transparent plastic vials. Chromatography vials should be sealed with screw caps to prevent contamination. Screw caps should have a center hole so that a septum can be inserted, while N9 screw caps have a threaded cap without a center hole. When purchasing N9 vials, you should purchase them from the same source, as replacement septa are sometimes unavailable. Another advantage of using ready-assembled closures is that they are automatically inserted and don't require manual assembly. Here is some more info about this service. A common type of chromatography vial is the crimp vial. Its name derives from the fact that it seals tightly with the septum between the glass vial and aluminum cap. Despite this, however, it requires the use of crimp tools to create a tight seal. In addition, snap caps don't require any tools for assembly, which makes them a good compromise between sealing strength and convenience. However, be aware that snap caps have the risk of cracking if they're not properly crimped. Other types of chromatography vials are available, including transparent, amber, and polypropylene. While both materials have their benefits, plastic is typically more economical and easy to handle. A glass-like appearance and a high degree of transparency make polypropylene a good choice for use in chromatography experiments. Similarly, transparent or amber borosilicate vials are compatible with HPLC instruments. For trace analysis in chromatography, type I borosilicate glass inserts are typically used. If your samples are pH-sensitive, you can also use polypropylene inserts. However, if you choose the wrong type of borosilicate glass insert for your chromatography vials, you may experience poor reproducibility between sample injections. Additionally, you risk damaging the needle used for autosampling. In addition to being easy to handle, screw thread closures are generally the most common choice. These are compatible with most autosamplers and offer a wide selection of cap and septum materials. They also meet analytical purity requirements. And as a result, they are safe for penetration. Crimp closures are another option if you're looking for an easy way to close chromatography vials. This type of closure is more convenient for autosamplers and is universally compatible. Discover more about the above topic by clicking here: https://en.wikipedia.org/wiki/Chromatography.
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